FIG. 6.
(A) DGGE band pattern obtained with the pmoA primer set targeting the gene of the α-subunit of the pMMO. For soil without CH4 oxidation (lanes dry soil to 2d) 35 μl (∼181 ± 59 ng) of product was loaded into each lane, and for soil with CH4 oxidation (lanes 4d to 23d) 35 μl (∼707 ± 203 ng) of PCR product was loaded into each lane. Mc, Methylococcus; Ms, Methylosinus; Mcs, Methylocystis. (B) Phylogenetic tree based on derived amino acid sequences of pmoA fragments, showing the relationship of the labeled DGGE bands to pmoA sequences of other methanotrophs. The sequences of the individual bands were obtained from the following lanes: band I, lanes dry soil, 9d control, 13d control, 4d, and 7d; bands II and III, lanes 7d and 14d; and bands IV through VI, lane 7d. The scale bar indicates the estimated number of changes per amino acid sequence position. Bootstrap values are given for 1,000 replicate trees.