FIG. 3.
(A) DGGE band pattern obtained with the universal SSU rDNA primer set targeting all life. PCR product (20 μl; ∼160 ± 41 ng) was loaded into each lane for all soil samples. The marked bands were sequenced. Mcs, Methylocystis; Ms, Methylosinus. (B) Phylogenetic tree constructed with partial 16S rDNA sequences, showing the relationship of the labeled DGGE bands to closely related members of the Bacteria. The sequences of the individual bands were obtained from the following lanes: band I, lanes 9d control, 4d, and 14d; band II, lanes 9d control and 14d; band III, lane 9d control; band IV, lane 4d; and band V, lane 9d control. The scale bar indicates the estimated number of base changes per nucleotide sequence position.