FIG. 3.
Southern blot analysis showing the effect of pNZRep-1 on intracellular Tuc2009 DNA replication. Lanes: 1, positive control (RsaI digest of Tuc2009 genomic DNA); 2 to 7, total cellular DNA, isolated at 20-min intervals, from Tuc2009-infected UC509.9 harboring pNZ8048 (lane 2, T = 0); 8, blank; 9 to 14, total cellular DNA, isolated at 20-min intervals, from Tuc2009-infected UC509.9 harboring pNZRep-1 (lane 9, T = 0). Tuc2009 was added at a multiplicity of infection of approximately 0.1. The blot was probed with a 1.9-kb PCR-generated Tuc2009 DNA fragment.