TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | F′::Tn10 proA+ B+ lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17(rK− mK+) supE44 relA1 lac | New England Biolabs |
| H. influenzae Rd KW20 | A. Wright | |
| Plasmids | ||
| pACYC177 | Kanr Apr | 5 |
| pACYC184 | Cmr Tetr | 25 |
| pJR207 | pLG339, Kanr, Tn10d-bla | 23 |
| pJRP4 | pACYC184, Cmr, hel | 24 |
| pAK1 | pACYC177, Kanr Aps | This work |
| pAK2 | pAK1, Kanr Aps, Tn10d-bla | This work |
| pAKbla | pACYC184, Cmr, Tn10d-bla | This work |
| pAKcat | pACYC177, Kanr Cmr Tn10d-cat | This work |
| pAKlacZcat | pACYC177, Kanr Cmr, Tn10d-lacZcat | This work |