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Illustrations of expected EDTA treated intestinal organoids images
Illustrations of bright field, HOECHST, green background autofluorescence (AF), and propidium iodide (PI) images of intestinal organoids treated with EDTA over time. In the PI images, the luminal areas of intestinal organoids are highlighted, which are made by selecting the luminal region in the green background AF. Analyzing PI stain over time in these luminal regions are indicative of organoid permeability.
