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IC50 determinations for the different inhibitors. The residual enzymatic activity was measured as a function of antibody fragment concentration. For all measurements, 100 μM nitrocefin was used as substrate. This was performed for cAbBCII10 (A), cAbTem02 (B), and cAbTem13 (C). For the experiment shown in panel A, 0.2 μM BcII was used, whereas 0.05 μM TEM-1 was used for the experiments shown in panels B and C.
