TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| M. smegmatis strains | ||
| mc2155 | Epta mutant of mc26, the wild-type orange rough clone from ATCC 607 | 18 |
| M24 | Mycobactin mutant of mc2155 | This study |
| B1 | mc2155 ΔfxbA Kmrb | This study |
| B3 | M24 ΔfxbA Kmr | This study |
| U3 | M24 ΔfxuA Kmr | This study |
| Plasmids | ||
| pUC4K | Kmr | Pharmacia |
| pGS22 | pUC118 containing a part of fxuC and fxuB on both ends of the aph gene from pUC4K | This study |
| pGS31 | pUC118 containing fxuB and a chromosomal fragment upstream of fxbA on both ends of the aph gene from pUC4K | This study |
a
Ept, efficient plasmid transformation phenotype.
b
Kmr, kanamycin resistance.