FIG 4.
Guinea pigs challenged with wild-type MACV after inoculation with MACV GPCΔN83/ΔN166/F438I. (A) Survival rate of animals inoculated with MACV GPCΔN83/ΔN166/F438I (n = 7) or naïve animals (n = 6) challenged 60 days postinoculation with an intraperitoneal injection of 104 PFU of wild-type MACV (Chicava). Data are representative of two independent experiments. (B) Morbidity rate of animals after infection. Data are representative of two independent experiments. (C) Body weight changes were monitored daily for the first 30 days postinfection. Error bars represent the SEM. Data are pooled from two independent experiments. (D) Temperature was monitored via BMDS transponder daily for 30 days postinfection. Error bars represent the SEM. Data are pooled from two independent experiments. (E) Virus titers in brain, liver, lung, spleen, and kidney. The minimum detection limit is shown as a dashed line. Data under detection limit is plotted as 1 PFU/g or mL. The solid bar represents the mean of the titers.