Table 1.

Transcription and Histone H3 Acetylation of the Coding Region in Several Genes

	RNA Pol II	K9-orf	K14-orf	K18-orf	K23-orf	K27-orf	Average
FAS1	92.0 ± 6.0	66.3 ± 6.1	46.7 ± 6.7	73.2 ± 33.2	42.8 ± 7.1	29.0 ± 4.0	51.6
FAB1	82.0 ± 9.3	21.6 ± 2.0	45.2 ± 9.7	53.9 ± 4.6	41.0 ± 3.9	38.9 ± 2.5	40.1
TRA1	80.2 ± 11.2	32.0 ± 8.1	58.4 ± 18.8	89.4 ± 30.0	50.7 ± 7.7	39.2 ± 0.5	53.9
POL2	64.9 ± 4.5	36.6 ± 5.2	56.8 ± 11.2	35.3 ± 7.1	31.7 ± 5.6	20.9 ± 0.5	36.2
GCN1	61.8 ± 6.4	29.3 ± 1.4	55.0 ± 8.1	71.3 ± 26.5	33.8 ± 6.2	25.3 ± 5.1	42.9
CDC39	53.1 ± 7.1	24.8 ± 2.9	69.4 ± 21.0	70.3 ± 19.6	41.0 ± 11.1	30.2 ± 2.7	47.0
ACT1	38.1 ± 9.1	27.1 ± 2.4	115.5 ± 20.8	14.7 ± 7.3	78.1 ± 6.2	23.7 ± 2.3	51.8
HXK1	37.0 ± 3.4	22.8 ± 5.2	76.4 ± 6.6	24.9 ± 2.0	67.1 ± 5.2	20.8 ± 1.7	42.4
PKC1	34.9 ± 8.9	24.0 ± 1.7	34.4 ± 5.5	37.5 ± 8.4	36.1 ± 11.2	24.5 ± 6.1	31.3
STE6	34.8 ± 3.4	24.3 ± 1.4	66.2 ± 1.6	41.2 ± 5.3	48.5 ± 3.2	15.1 ± 0.5	39.1
GDH1	27.3 ± 6.4	21.0 ± 1.1	26.7 ± 8.7	50.7 ± 3.0	18.5 ± 0.5	15.6 ± 1.3	26.5
SSA4	27.0 ± 3.0	20.1 ± 0.8	40.2 ± 8.0	32.2 ± 2.3	27.8 ± 0.2	12.5 ± 0.4	26.6
YGP1	26.2 ± 3.4	21.2 ± 0.5	36.1 ± 10.1	24.0 ± 2.0	37.1 ± 11.1	11.9 ± 2.4	26.1
BAT1	21.8 ± 2.0	23.0 ± 1.3	34.4 ± 15.4	23.6 ± 8.5	19.9 ± 3.4	13.1 ± 0.2	22.8

ChIP analyses of transcription (RNAPII density) and the relative level of acetylated histone H3 in the coding region of 14 different genes using antibodies directed towards RNAPII and specific, individual acetylation sites. The numbers shown are for gcn5 elp3 cells in percent of wild-type and are averages (with standard deviations) of at least three independent experiments. The column to the right shows the average acetylation of the gene. Lysine residues showing severe hypoacetylation (less than 25% of wild-type) are underlined.