Figure 2.
Near-single-cell spatial resolution in diabetic mouse kidney reveals an expansion of granular cells and a disrupted blood flow regulating apparatus
(A) Arrays displaying cell types in BTBR w/w (left) and BTBR ob/ob diabetic mice (right). Images of individual cell populations are plotted in Figure S64. Scale bars, 500 μm.
(B–F) Plots from Slide-seq arrays showing (B) average area of glomeruli (p value <0.0001), (C) percentage of beads classified as podocytes, (D) percentage of beads classified as glomerular endothelial cells, (E) percentage of beads classified as glomerular mesangial cells, (F) percentage of beads classified as granular cells (p value 0.0009) in BTBR w/w and BTBR ob/ob mice.
(G) Plot from Slide-seq arrays showing the average distance between the center of glomerulus and granular cell cluster in BTBR w/w and BTBR ob/ob mice (p value 0.0018). Data were obtained from cross-sections of four mice per genotype.
(H) HCR validation images, showing Nphs2+ podocytes, Ren1+ granular cells, Slc12a1+ TAL, and all cells in DAPI. Scale bar, 50 μm. Disorganized Ren1+ cells are denoted with white arrows.
(I and J) HCR quantification of (p value <0.0001) and (J) HCR images of Ctgf + Nphs2+ injured podocytes (arrows). Scale bar, 50 μm. Data obtained from entire cross sections of a kidney from four mice per genotype. All error bars represent standard deviation of the mean.