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. Author manuscript; available in PMC: 2022 Nov 22.
Published in final edited form as: Curr Biol. 2021 Sep 22;31(22):4898–4910.e4. doi: 10.1016/j.cub.2021.09.004

Figure 5: Purifications and TMT-MS analysis of endogenously tagged human spliceosomal protein orthologs.

Figure 5:

A. Schematic of sample preparation for TMT-MS. Shown are relative normalized abundances of the sum of the low- and high-salt peptide intensities of the spliceosomal protein orthologs.

B. IP-MS results for 2x-FLAG GPATCH1. Plotted are TMT-MS data with length-normalized peptide intensity (log10) on the Y-axis and rank on the X-axis. See also Data S3.

C. IP-MS results for 2x-FLAG RBM17. Plotted are TMT-MS data with length-normalized peptide intensity (log10) on the Y-axis and rank on the X-axis. See also Data S4.

D. IP-MS results for 2x-FLAG RBM5. Plotted are TMT-MS data with length-normalized peptide on the Y-axis (log10) and rank on the X-axis. See also Data S5.