TABLE 1.
Bacterial strains, plasmids, and phage used for this study
| Strain, plasmid, or phage | Genotype or phenotype | Reference |
|---|---|---|
| E. coli K-12 | ||
| CC118 | araD139 Δ(ara-leu)7697 argE(Am) galE galK ΔlacX74 ΔphoA20 recA1 rpoB rpsE thi | 24 |
| RYC1000 | araD139 gyrA ΔlacU169 rbsΔ7 recA56 relA rpsL thiA | 10 |
| CM1470 recA | asnB32 atp-706(ΔIBEFHA) recA relA1 spoT1 thi-1 | 34 |
| Plasmids | ||
| pEX2000 | pBR322 carrying the MccH47 system | 10 |
| pEX4 | pUC13 carrying the MccH47 system | 21 |
| pEX4::Tn5 76 | pEX4 with Tn5 in mchB | 10 |
| pUY69 | pUC13 carrying mchI | 27 |
| pMVD10 | pACYC184 carrying the mchB72-phoA gene fusion | This work |
| pUY100 | pUC13 carrying mchB | This work |
| Phage | ||
| λ-TnphoA | b221 cI857 Pam3 with TnphoA in or near rex | 15 |