Figure 4.
VRC01gHL BGC cells acquire rare mutations, including L-CDR1 deletions
(A) Clones with an L-CDR1 deletion after immunization with the MD39-GT3.1 trimer (M8, M12, M14, M15) or eOD-GT5 60-mer (E11). n is the total number of sequences analyzed. The three day 36 clones are derived from different mice. M14 and M15 may or may not be from the same mouse.
(B) Mutation cold spots (blue) and hotspots (orange) in L-CDR1 of IGKV3-11.
(C and D) ARMADiLLO analysis of mutated day 36 HC (C) and LC (D) sequences from MD39-GT3.1-immunized mice, showing the fraction of sequences that acquired the given number of improbable mutations.
(E) Codon changes (orange) required for a threonine-to-valine/isoleucine mutation.
(F) Antibodies selected for expression and their mutation profiles.