FIG. 3.
Phylogenetic relationships of TTV with the animal circoviruses CAV, porcine circovirus (PCV), and psittacine beak and feather disease virus (BFDV). The phylogenetic tree was constructed by comparing the entire deduced products of the ORFs that contain Rep protein motifs, namely, ORF1 (TTV), VP1 (CAV), ORF1 (PCV), V1 (BFDV), and VP1 (parvovirus B19), using the last as outgroup. TTV isolates are grouped based on N22 segment analysis 106, 120. The Fitch-Margoliash algorithm was used by randomizing the input order of sequences. Genetic distances were calculated by using the Poisson correction distance (PoissonP) as provided by DAMBE software (version 3.7.48) 182. Bootstrap values above 70 out of 100 are shown at the main branch points. The tree was drawn by using the Treeview program (version 1.5.2) 129. The bar indicates the number of amino acid substitutions per site. All sequences are available in the GenBank database and are indicated by their accession number or, whenever possible, isolate name.