TABLE 1.
Yeast strains and plasmids used in this study
| Designation | Genotype and comments | Reference |
|---|---|---|
| Strains | ||
| NOY408-1b | MATa ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 pNOY102 | 23 |
| TAK200 | Same as NOY408-1b except for deletion of rDNA repeats, leaving two copies (Fig. 3), and for the presence of pRDN-hyg1 instead of pNOY102 | |
| TAK201 | Same as TAK200 except for the presence of fob1Δ::HIS3, and pNOY353 instead of pRDN-hyg1 | |
| Plasmids | ||
| pRDN-hyg1 | Multicopy plasmid carrying rdn-hyg1, rdn-ani1, leu2-d, URA3, 2 μm, and Ampr | 4 |
| pNOY353 | Multicopy plasmid carrying GAL7-35S rDNA, 5S rDNA, TRP1, 2 μm, and Ampr | 24 |
| YEplac181 | Multicopy plasmid vector carrying LEU2 and 2 μm | 8 |
| pTAK101 | YEplac181 carrying FOB1 |