FIG. 7.
Western blot analysis of tissue extracts from wild-type (+/+) and homozygous (−/−) animals. Tissue extracts from heart (H), lung (L), skeletal muscle (M), and kidney (K) were separated by SDS-PAGE under nonreducing conditions for detection of nidogen 2 (A) and under reduced conditions for detection of laminin chains (B). The blots were probed with antibodies directed against nidogen 2 or the laminin 1-nidogen 1 complex. Calibration shown on the right was carried out with globular proteins (in kilodaltons).