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. 2022 Feb 22;22:93. doi: 10.1186/s12935-022-02490-5

Fig. 4.

Fig. 4

LINC01128 was a sponge for miR-561-5p. a The subcellular location of LINC01128 was determined by subcellular fractionation assay. b Sequences of WT-LINC01128, Mut-LINC01128, and miR-561-5p. c Luciferase assay of SW1990 and BxPC-3 cells transfected with LINC01128-MUT or LINC01128-WT reporter gene, together with NC or miR-561-5p. d MS2-RIP was applied to evaluate endogenous miR-561-5p associated with the MS2-tagged LINC01128. e The expression level of miR-561-5p in BxPC-3 and SW1990 cells. f The expression level of miR-561-5p in PC tissues and adjacent normal tissues. g The correlation between LINC01128 and miR-561-5p levels was determined by Spearman's rank correlation test analysis. h, i CCK-8 assays and transwell assays were used to detect the proliferation, migration, and invasion ability. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01