Fig. 1. Study design and single-cell transcriptional profiling of lung cells.
A H&E stanning of biopsy specimens in the left upper lobe from the patient with FHP showing massive fibroblast foci (black star) and mono-nuclear cell infiltration (black triangle). Scale bar = 20 μm. B Schematic of the overall study design. The scRNA-seq and scTCR-seq were applied to surgical lung biopsy specimens of the FHP patient, and the output data were integrated with single-cell transcriptome data from an FHP patient and two transplant donors in a publicly available dataset for analysis. C t-SNE visualization of 29 cell clusters. D t-SNE visualization of epithelial cells, macrophages, endothelial cells, dendritic cells, fibroblasts, T cells, B cells, NK cells, mast cells, and monocytes. DC dendritic cells. E The t-SNE map showing the distribution of cells between control and FHP group. F Relative percentage of sample origins across cell types. G–I AUCell analyses of the relative gene set enrichment scores in Pro-inflammation signature (G), Cytokines/Chemokines/Receptors signature (H), and EMT (I) signature. The t-SNE maps showing AUC scores of selected gene signatures (left); the box plots showing AUC scores of selected gene signatures in each cell type (middle) or sample group (right). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.