Fig. 8.
Alleviation of hippocampal neuron damage and inhibition of abnormal microglia activation by TT-NM/Rapa. (A) Western blot analysis of synaptophysin (SYAP) protein in the brain of 3 × Tg-AD mice treated with various rapamycin formulations or saline. (n = 3). (B) Representative images of Nissl staining of hippocampal region from 3 × Tg-AD mice treated with various rapamycin formulations or saline. Image in lower row of each group were the enlarged regions of the black boxes in the upper images. (C) Quantitative analysis of the number of neurons in the entire hippocampal region of Fig. 8B by Image J. (n = 3). (D) Representative confocal images of immunofluorescence staining of Iba1 in cortical region from 3 × Tg-AD mice treated with various rapamycin preparations or saline. Green, Iba1-stained activated microglia; Blue, nuclei stained by Hoechst 33,342. (A) and (C) Data were presented as mean ± SD. #p < 0.05, ##p < 0.01, compared with AD group; *p < 0.05, **p < 0.01, between the compared groups.