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Analysis of major PDGF signaling pathways in ferD743R embryonic fibroblasts. Wild-type (+/+), heterozygous (+/−), and homozygous (−/−) ferD743R cells were starved and stimulated with PDGF-BB for 5 min. Soluble cell lysates were separated by SDS-PAGE and blotted for tyrosine-phosphorylated proteins (pY), Fer, phospho-Akt (pAkt), Akt, phospho-Stat3 (pStat3), Stat3, phospho-Erk1/2 (pErk1/2), and Erk1/2. Relative molecular weights are indicated in thousands on the left.
