FIG. 2.
Analysis of Fer expression and kinase activity in ferD743R mice. (A) Liver homogenates from wild-type (+/+), heterozygous (+/−), and homozygous (−/−) ferD743R mice (strain 153) were subjected to immunoprecipitation (IP) with Fer antisera, followed by in vitro kinase reactions. The position of Fer is indicated on the left. (B) Testis homogenates from +/+, +/−, or −/− ferD743R male mice were subjected to immunoprecipitation with Fps/Fer antisera (raised against the SH2 domain of Fps but cross-reactive with Fer and FerT), followed by in vitro kinase reactions. The positions of Fer, FerT, and the IgG heavy chain are indicated on the left. Cross-reacting bands in the soluble cell lysate, and a putative partial degradation product in the kinase assay, are indicated with asterisks.