FIG. 7.
The C-terminal region of BAF250 directly interacts with GR. (a) Graph showing that deletion of the C-terminal region of BAF250 decreases its ability to facilitate GR-dependent gene activation. The assays were performed in the presence (shaded bars) or absence (black bars) of DEX (10−7 M). Schematic diagrams of the full-length BAF250 and deletion mutants are shown below. (b) Immunoblot analysis of extract from T47D cells transfected with full-length BAF250 or a C-terminal deletion mutant. The arrows mark each protein. A polypeptide cross-reactive with the antibody (∗) was used as a loading control. The nuclear extract from KB cells was the positive control (lane 1). (c) Autoradiograph showing that the C-terminal region of BAF250 directly interacts with GR in a GST pull-down assay. Either GST alone or GST fused to the C-terminal region of BAF250 (amino acid residues 1670 to 2137) was used. The presence or absence of DEX (10−7 M) is indicated. The 35S-labeled GR protein was produced using an in vitro transcription and translation system.