FIG. 4.
BAF250 distinguishes human BAF complex from PBAF. (a) Silver-stained SDS-PAGE gel of human BAF complex purified by anti-BRG1 (lane 1) or anti-BAF250 antibody (lane 2). The PBAF complex is shown as a comparison (lane 3). Lines, subunits shared by both complexes; arrows, components unique to each complex. (b) Western blotting of the load, flowthrough (FT), and eluate fractions from the BAF250 antibody column. (c) Autoradiograph showing the mononucleosome disruption activity by human BAF complex purified with a BAF250 antibody (lanes 4 and 5). The results for the BAF complex purified with a BAF57 antibody are also shown for comparison (lanes 6 and 7). The templates and the complexes used in each reaction are shown at the top. The presence (+) or absence (−) of ATP is indicated. C, control in which antibody beads without the complex loaded was tested.