Figure 3 – PVN pro-inflammatory cytokine density.

Example confocal maximum projection images of PVN TNF-α (A) and IL-6 (B) immunofluorescence (white) with respective anatomic marker, vasopressin (insets; red) in WKY, SHR, SHR-Los, and SHR-TAK (n=6/group). Box-plot diagrams of TNF-α (C) and IL-6 (D) protein density in the PVN, calculated as change in % area relative to WKY. Data evaluated by one-way ANOVA with Tukey post-hoc analysis; shown as mean±SEM; ****p<0.0001 vs. WKY; +++p<0.001 vs. SHR; ++++p<0.0001 vs. SHR; scale bars: 200μm; 3V: third ventricle.