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. 2019 Feb 26;3(1):e000035. doi: 10.1136/bmjos-2017-000035

Table 6.

Secondary outcome: statistical items, in vivo and in vitro experiments

Item NPG before NPG after Adj p Non-NPG before Non-NPG after Adj p
n N % CI n N % CI n N % CI n N % CI
In vivo
Exact n 93 203 45.8 39.8 to 52.7 131 189 69.3 62.4 to 75.5 0.0004 76 164 46.3 38.8 to 54.0 89 189 47.1 40.1 to 54.2 NS
Technical or biological replicates 137 201 68.2 61.4 to 74.2 151 188 80.3 74.0 to 85.4 NS 92 164 56.1 48.4 to 63.5 102 188 54.3 47.1 to 61.2 NS
Number of times replicated 45 199 22.6 17.3 to 28.9 68 183 37.2 30.5 to 44.4 NS 35 164 21.3 15.7 to 28.3 36 188 19.1 14.1 to 25.4 NS
Test described if uncommon? 26 51 51.0 37.5 to 64.3 41 68 60.3 48.3 to 71.7 NS 24 42 57.1 41.1 to 71.9 30 53 56.6 42.4 to 69.9 NS
t-Test defined as one-sided or two-sided? 61 133 45.9 37.6 to 54.4 102 144 70.8 62.9 to 77.7 0.003 30 100 30.0 21.8 to 39.7 50 109 45.9 36.8 to 55.3 NS
Correction for multiplicity 63 116 54.3 45.2 to 63.1 69 122 56.6 47.6 to 65.1 NS 57 101 56.4 46.6 to 65.8 74 118 62.7 53.7 to 71.0 NS
Reporting full statistics 39 167 23.4 17.6 to 30.4 36 171 21.1 15.6 to 27.8 NS 21 136 15.4 10.3 to 22.5 20 156 12.8 8.4 to 19.0 NS
Reporting of average 135 181 74.6 67.7 to 80.4 147 171 86.0 79.1 to 90.4 NS 110 147 74.8 67.2 to 81.2 121 168 72.0 64.8 to 78.3 NS
Definition of error bars 159 181 87.8 82.2 to 91.9 155 168 92.3 87.1 to 95.4 NS 118 144 81.9 74.8 to 87.4 129 165 78.2 71.2 to 83.8 NS
Testing of assumptions 15 171 8.8 5.4 to 14.0 44 165 26.7 20.5 to 33.9 0.002 12 134 9.0 5.2 to 15.1 18 156 11.5 7.4 to 17.6 NS
Reporting measures of variation 143 183 78.1 71.6 to 83.5 139 172 80.8 74.2 to 86.0 NS 115 142 81.0 73.4 to 86.9 125 169 74.0 66.6 to 80.3 NS
Variation less than twofold 13 53 24.5 14.8 to 37.8 22 61 36.1 25.1 to 48.8 NS 12 49 24.5 14.5 to 38.4 17 50 34.0 22.3 to 48.0 NS
In vitro
Exact n 51 158 32.3 25.4 to 40.0 93 181 70.5 44.1 to 58.6 0.050 49 133 36.8 29.1 to 45.4 43 165 26.1 19.9 to 33.3 NS
Technical or biological replicates 90 159 56.6 48.8 to 64.1 137 182 75.3 68.5 to 81.0 0.035 52 133 39.1 31.2 to 47.6 70 165 42.4 35.1 to 50.1 NS
Number of times replicated 62 158 39.2 31.9 to 47.1 85 181 47.0 39.8 to 54.2 NS 48 132 36.4 28.6 to 44.9 58 164 35.4 28.4 to 43.0 NS
Test described if uncommon? 23 42 54.8 39.7 to 69.0 36 58 62.1 49.0 to 73.6 NS 19 44 43.2 29.5 to 58.0 14 39 35.9 22.6 to 51.9 NS
t-Test defined as one-sided or two-sided? 51 109 46.8 37.6 to 56.2 94 130 72.3 64.0 to 79.3 0.008 27 82 32.9 23.6 to 43.8 30 89 33.7 24.7 to 44.1 NS
Correction for multiplicity 48 102 47.1 37.6 to 56.7 59 114 51.8 42.6 to 60.8 NS 40 95 42.1 36.7 to 57.6 54 100 54.0 44.2 to 63.5 NS
Reporting full statistics 25 140 17.9 12.4 to 25.1 27 160 16.9 11.8 to 23.5 NS 16 112 14.3 8.9 to 22.0 19 135 14.1 9.2 to 21.0 NS
Reporting of average 122 149 81.9 74.5 to 87.3 140 160 87.5 81.4 to 91.8 NS 90 126 71.4 62.9 to 78.6 111 148 75.0 67.4 to 81.3 NS
Definition of error bars 136 149 91.3 85.6 to 94.9 155 164 94.5 89.8 to 97.1 NS 98 122 80.3 72.3 to 86.4 119 146 81.5 74.4 to 87.0 NS
Testing of assumptions 13 139 9.4 5.5 to 15.4 34 155 21.9 16.1 to 29.1 NS NS 110 6.4 3.1 to 12.8 9 133 6.8 3.6 to 12.5 NS
Reporting measures of variation 112 149 75.2 67.6 to 81.4 132 162 81.5 74.8 to 86.7 NS 89 125 71.2 62.7 to 78.4 103 147 70.1 62.1 to 76.9 NS
Variation less than twofold 9 40 18.4 9.8 to 31.7 15 55 27.3 17.2 to 40.4 NS 12 46 26.1 15.4 to 40.5 17 45 37.8 24.9 to 52.6 NS

n, number meeting the criteria; N, total number of studies where that criteria is relevant; %, per cent meeting the criteria; CI, 95% CI of that percentage; Adj p, adjusted significance level (two-sample proportion test (prop.test) followed by Holm-Bonferroni correction (p.adjust.methods)); NS, not significant at p<0.05.

NPG, Nature Publishing Group.