FIG. 5.
The ski4-1 mutation is complemented by a wild-type (WT) CSL4 gene. (A) Plates of YEP containing either 2% glucose or 2% Gal and 2% sucrose. The GAL::csl4 strain fails to grow on plates containing glucose but grows on plates containing Gal and sucrose, because the only copy of the essential CSL4 gene has been placed under the control of the Gal promoter. This conditional growth defect is corrected by pCSL4. (B) Polyacrylamide Northern blot of RNA from wild-type and ski4-1 strains containing either pCSL4 or the empty vector. The blot was probed with an oligonucleotide that detects the poly(G)-to-3′-end fragment of PGK1pG mRNA. The detected species are indicated with cartoons on the right. The length of HpaII fragments of pUC18 is givne in nucleotides. M, nucleotide marker lane. (C) Plates containing YEP–2% glucose. The indicated strains were grown at 33°C.