Figure 6.
Effect of amputation location on Hox gene expression patterns. Schematic summarizing Hox gene expression patterns in uncut animals and animals amputated at different segmental positions. (A) Genomic organization of 10 of the Capitella Hox genes present in the Capitella genome [59]. Colored rectangles indicate position of coding sequence and black line indicates genomic linkage. (B–F) Schematics of animals with representation of Hox gene expression patterns. Anterior is to the left and the posterior growth zone (pgz) is indicated. Segment number is indicated above animal in B. Colored bars indicate position of Hox gene expression along the anterior–posterior axis. Color coding matches that of genes shown in (A,B). Uncut animals show staggered anterior Hox gene expression boundaries. (C–F) Depiction of expression boundaries 24 hours after amputation at specific segmental positions. Amputations were performed at the boundary between segments 7 and 8 (C), 8 and 9 (D), 9 and 10 (E) and 10 and 11 (F). Black arrows indicate examples of shifts in anterior gene expression boundaries in the anterior direction relative to expression boundaries in uncut animals. Adapted from [19].