Figure 5.
The central nervous system and regeneration. All panels are in ventral view with anterior to the left. Vertical dotted lines indicate approximate position of amputation site. (A–C) are confocal projections. Nerves are shown by acetylated tubulin reactivity (acet-tub+) in (A–C). (A,B) Arrows indicate extension of neurites (red) into the regenerating tissue. Green indicates incorporation of EdU. (C) Regeneration of posterior segments in the absence of brain and anterior segments. White arrowheads indicate regenerated ganglia. Nerves are indicated in white and nuclei in blue (Hoechst 33342 staining). (D) Scheme of double amputations to assess role of brain during posterior regeneration. Dotted areas show regions of body removed during amputation to generate image in C. Images are adapted from [38]. The time following amputation (in days) is indicated in bottom left of panel (dpa).