FIG. 4.
ARF6 effector domain includes residues Q37 and S38. (Top) Amino acid sequence comparison between human ARF1 and ARF6 from amino acid 24 in ARF1 (20 in ARF6) to amino acid 56 in ARF1 (52 in ARF6). Asterisks indicate identity. Note the conservation in the switch I region encompassing residues 45 to 54. (Bottom) HeLa cells were transfected with plasmids encoding either the 1-6 chimera, 1-6(EI→QS), ARF6, or ARF6(QS→EI). Cells were then left untreated or incubated for 30 min with AlF. The expressed proteins were labeled with ARF6-specific antiserum followed by rhodamine-conjugated phalloidin to visualize F-actin. Bar, 15 μm.