TABLE 1.
End-to-end fusions observed in the mTER+/+ and mTER−/− ES cells
| Sample and no. of PDL | No. of:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Metaphases analyzed | Chromosomes per metaphase | Fused chromosomesa | Fusionsa | Fused chromosomesb
|
Fragmentsa | ||||
| RLC | Dic/Tric | Rings | Others | ||||||
| E14 mTER+/+ cells | |||||||||
| 10 | 17 | 40.59 ± 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1,134 | 18 | 40.39 ± 0.4 | 3 (0.17) | 3 | 3 (100) | 0 | 0 | 0 | 0 |
| mTER−/− cells | |||||||||
| DKO301 | |||||||||
| 142 | 20 | 40.95 ± 0.4 | 29 (1.5) | 29 (1.5) | 25 (86.2) | 0 (0) | 3 (10.3) | 1 (3.5) | 0 (0) |
| 457c | 16 | 30.31 ± 2.6 | 260 (16.3) | 283 (17.7) | 207 (79.6) | 17 (6.5) | 16 (6.2) | 20 (7.7) | 8 (0.5) |
| 465c | 17 | 26.94 ± 0.8 | 188 (11.1) | 286 (16.8) | 141 (75.0) | 37 (19.7) | 4 (2.1) | 6 (3.2) | 0 (0) |
| 692 | 19 | 22.92 ± 0.5 | 362 (19.1) | 379 (20.0) | 315 (87.0) | 33 (9.1) | 4 (1.1) | 10 (2.8) | 2 (0.1) |
| DKO741 | |||||||||
| 154 | 17 | 41.41 ± 0.8 | 21 (1.2) | 22 (1.3) | 12 (57.1) | 0 (0) | 8 (38.1) | 1 (4.8) | 5 (0.3) |
| 423 | 19 | 26.16 ± 1.4 | 259 (13.6) | 342 (18.0 | 213 (82.2) | 34 (13.1) | 8 (3.1) | 4 (1.54) | 8 (0.4) |
| 435c | 15 | 24.20 ± 2.3 | 224 (14.9) | 274 (18.3) | 190 (84.8) | 18 (8.0) | 13 (5.8) | 3 (1.3) | 2 (0.1) |
| 683 | 18 | 25.50 ± 1.9 | 477 (26.5) | 598 (33.2) | 439 (92.0) | 31 (6.5) | 5 (1.1) | 2 (0.4) | 1 (0.1) |
a
Numbers in parentheses indicate frequency per metaphase.
b
Numbers in parentheses indicate percentage of total fusions observed. RLC, Robertsonian fusion like configurations; Dic/tric, dicentrics/tricentrics. Other fusions include q-p fusions and complex fusions.
c
Growth crisis PDL.