FIG. 6.
C'SMRT can partially reverse hPRA-mediated repression of hER transcriptional activity. HeLa cells were transiently transfected with 1 μg of 3XERE-TATA-LUC, 50 ng of pBKC-βgal, 450 ng of pRST7-ERα, 300 ng of pBKC-hPRA, and increasing concentrations (ranging from 0 to 1.2 μg) of either Gal4-SMRT (A), Gal4-C'SMRT (B), or ΔN4, used as a control (C). Various amounts of pBKC-DBD were added to balance the amount of input Gal4DBD. Transcriptional activity of the 3XERE-TATA-LUC reporter was measured 24 h after the addition of 10−7 M 17-β-estradiol and 10−7 M RU486. A control was done in the absence of ligands (not shown). The data are presented as percent activation where 100% represents a measure of 17-β-estradiol-dependent transactivation by hER in the absence of RU486 (Cont). The average coefficient of variation at each point was <12% (n = 2).