Extended Data Table 1 |.
Statistics for data collection and structural refinement
| Cav2.2-ziconotide (EMDB-23867) (PDB 7MIX) |
Cav2.2-apo (EMDB-23868) (PDB 7MIY) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | 105,000 |
| Voltage (kV) | 300 | 300 |
| Electron dose (e-/Å2) | 50 | 50 |
| Defocus range (μm) | −2.1~−1.9 | −2.1~−1.9 |
| Pixel size (Å) | 1.114 | 1.114 |
| Symmetry | C1 | C1 |
| Initial particle images (no.) | 1,909,156 | 928,665 |
| Final particle images (no.) | 170,839 | 56,616 |
| Map resolution (Å) | 3.0 | 3.1 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 2.8-31.2 | 2.9-50 |
| Refinement | ||
| Initial model used (PDB code) | 5GJW, 1VYU, 1OMG | Cav2.2-ziconotide |
| Model resolution (Å) | 3.3 | 3.4 |
| FSC threshold | 0.5 | 0.5 |
| Map sharpening B factor (Å2) | −79 | −59 |
| Model composition | ||
| Non-hydrogen atoms | 21654 | 21485 |
| Protein residues | 2623 | 2598 |
| Ligands | 28 | 28 |
| B factors (Å2) | ||
| Protein | 58.27 | 78.42 |
| Ligand | 69.59 | 62.16 |
| R.m.s deviations | ||
| Bond lengths (Å) | 0.004 | 0.003 |
| Bond angles (°) | 0.759 | 0.727 |
| Validation | ||
| MolProbity score | 2.00 | 1.95 |
| Clashscore | 9.72 | 9.68 |
| Poor rotamers (%) | 1.47 | 1.31 |
| Ramachandran plot | ||
| Favored (%) | 94.76 | 94.99 |
| Allowed (%) | 5.12 | 4.86 |
| Disallowed (%) | 0.12 | 0.16 |