Extended Data Fig. 2 |. Cryo-EM structure of the human Cav2.2 complex bound with ziconotide.
a, Heat map for local resolution of the complex. The resolution map was calculated in Relion 3.0 and prepared in Chimera. Top inset, bound ziconotide (labelled as Zi) is well-resolved. Bottom inset, resolution of the β3 subunit, after a focused refinement, allows for reliable model building using the crystal structure of rat β3 (PDB code 1VYU) as a template. b, Overall structure of the complex at an averaged resolution of 3.0 Å. Left, the complex comprises the α1 core subunit (silver), the extracellular α2δ−1 subunit (light pink for α2 and green for δ) and the cytosolic β3 subunit (wheat). The peptide pore blocker ziconotide is coloured brown. The resolved lipid, cholesterol and CHS molecules are shown as black sticks. The bound PIP2 is shown as black ball-and-sticks. Sugar moieties are shown as thin sticks. Right, surface presentation of the structure. The four repeats are coloured grey, cyan, yellow, and pale green. The III–IV linker and the CTD are coloured orange and pale purple, respectively.