FIG. 7.
Analysis of snR38 accumulation in the wt CH1462 and the mutant dbr1-Δ strains. Total RNA was extracted from strains CH1462 (left) and dbr1-Δ (right) carrying the pGAL38wt-tag and pGAL38ΔS-tag constructs at the indicated times of galactose induction (expressed in hours above the lanes; hr-gal). Total RNA (5 μg) was resolved on a 6% acrylamide–7 M urea gel, electroblotted onto a nylon membrane, and hybridized with the 38 anti-tag oligonucleotide. The different processing products are diagrammed. Lane M: pBR322 plasmid DNA, MspI digested.