FIG. 8.
Overexpression of PR48 alters cell cycle progression. Flow cytometry profiles of DNA content (propidium iodide fluorescence) and estimates of the percentage of cells in G1, S, and G2/M stages of the cell cycle in asynchronously growing HeLa cells under control conditions and following transfection to express either GFP, PR48-GFP, Bα-GFP, or B′α1-GFP. Profiles of cell number versus DNA content (DNA) were constructed from the cells transfected with an empty vector, pTarget (Vector), or selectively within the transfected cell population as identified by direct (GFP) fluorescence.