FIG. 6.
Comparison of myc-tagged Cln proteins. Wild-type strain (1255-5c) was transformed with plasmids pRS414 (vector, lane 1), pMM45 (Cln3, lane 2), pMM55 (Cln3-1, lane 3), pMM83 (NLS–Cln3-1, lane 4), pMM84 (mnls–Cln3-1, lane 5), pMM85 (NES–Cln3-1, lane 6), pMM86 (mnes–Cln3-1, lane 7), pMM82 (Cln2, lane 8), pMM60 (NLS-Cln2, lane 9), pMM61 (mnls-Cln2, lane 10), pMM92 (NES-Cln2, lane 11), and pMM93 (mnes-Cln2, lane 12). All plasmids are episomal CEN (low copy number) and express the myc-tagged Cln protein from the CLN3 promoter. Cellular lysates were separated by SDS–12% PAGE and analyzed by Western blotting as described in Materials and Methods.