FIG. 4.
Induction of oocyte maturation by S128D/T201D Plx1. Stage VI oocytes were microinjected with 40 ng of mRNA encoding either WT Plx1 or S128D/T201D Plx1, and groups of six oocytes were frozen at the indicated times. (A) Extracts were prepared, and histone H1 kinase activity was determined. Symbols: □, WT Plx1; ■, S128D/T201D Plx1. (B) Samples of extracts were subjected to immunoblotting with anti-Cdc25C, anti-phospho-MAP kinase, and anti-Mos antibodies as indicated. Upper panel of each pair, S128D/T201D Plx1 (mRNA injected); lower panel of each pair, WT Plx1 (mRNA injected).