Figure 4.
LOP triggers ultrastructural hallmarks of reticulophagy. (A-F) MZ-54 WT cells were left untreated (A-C) or treated with 17.5 µM LOP (D-F) for 48 h before being processed for electron microscopy. Scale bar: 1 µm (A and D) or 500 nm (b, c, e, and f). (G) The average number of ER+ and ER− autophagosomes per cell section was determined as described in Materials and Methods. Significances are calculated versus untreated cells. *** p < 0.001. A, autophagosome; D, degradative compartment; ER, endoplasmic reticulum; M, mitochondria; N, nucleus; PM, plasma membrane; UT, untreated; asterisk, rough-ER fragment