FIG. 2.
The AR interacts with coactivator proteins via the AF2 core. A two-hybrid assay was performed with yeast and coactivator fragments or full-length protein, as indicated, as bait. The activity of the lacZ reporter in the presence of the VP16 activation domain alone (hatched bars) or fused to the AR LBD without (black bars) or with (gray bars) androgen was measured, and values represent intrinsic activities of the coactivator and the ligand-dependent interaction with AR. NH, no hormone; MB, mibolerone.