FIG. 3.
The Mcm4,6,7 protein complex can bind single-stranded DNA. (A) Proteins in the gradient fractions were electrophoresed on a 5% native polyacrylamide gel and stained with silver. As markers, thyroglobulin (669 kDa) and ferritin (440 kDa) were electrophoresed. (B) A gel shift assay was carried out with the Mcm4,6,7 protein complexes present in the gradient fractions. 32P-labeled 37-mer oligonucleotides were incubated with the fractions. After cross-linking, DNA-protein complexes were separated by native PAGE (5% polyacrylamide). Autoradiography of the dried gel was performed.