FIG. 2.
Expression of cardiac-specific genes was detected in P19CL6 cells but not in P19CL6noggin cells. RNA was prepared from parental P19CL6 cells and P19CL6noggin cells on day 0 (before treatment with DMSO) (lanes 1 and 4), day 6 (lanes 2 and 5), and day 12 (lanes 3 and 6). RT-PCR was performed to analyze BMP-2, BMP-4, and Csx/Nkx-2.5 mRNA. Ten micrograms of RNA from each sample was subjected to Northern blot analysis for other genes. Ethidium bromide staining of rRNA is presented at the bottom to show that the same amount of intact RNA was loaded in each lane.