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Role of Cas in EGF-induced SRE activation. Cells were cotransfected with pSREtkLuc and either vector DNA (pSSRα) or constructs expressing Caswt or CasΔSB. Twenty hours after transfection, the cells were serum starved for 24 h. Cells were either left unstimulated or stimulated with EGF (10 ng/ml) for 5 h. The cells were lysed and luciferase activity determined. ∗, P = 0.01.
