Figure 8.
Tumor‐induced COX‐2 contributes to tumor‐mediated activation of Stat3/Stat1 in BM‐derived myeloid cells. BM cells from naïve BALB/c mice were cultured in the presence of mouse rGM‐CSF (20 ng/ml) or CT‐26 conditioned media (25% of total volume) in an ultra‐low attachment plate for 6 days. The COX‐2 inhibitor was added at a final concentration of 40 μM. On Day 6, cells were collected, washed in PBS, and fixed immediately in BD Phosflow lyse/fix buffer (BD Biosciences). Intracellular flow cytometric analysis of phospho‐Stat1 (pSTAT1; pY701) and phospho‐Stat3 (pSTAT3; pY705) was performed using BD Phosflow technology (BD Biosciences).