Figure 7.
The inhibition of COX‐2 activity in BM precursors partially restores the development of APCs. BM cells were cultured in the presence of GM‐CSF and/or CT‐26 TCM. Inhibitors of COX‐2 or mPGES1 were added on Day 0 at indicated concentrations. (A and B) On Day 8, cells were collected, and expression of CD11c and F4/80 was assessed using flow cytometry. Results of one representative experiment out of four are shown. (C) Effect of COX‐2 inhibitor on tumor‐induced arginase activity in myeloid cell lysates was evaluated using the quantitative colorimetric arginase assay kit as described in Materials and Methods. Average means ± sd are shown. (D) Cytospin slides were stained with H&E and analyzed under a light microscope. *P < 0.05.