Figure 5.
Exposure of BM‐derived myeloid cells to tumor‐derived factors promotes M2‐oriented cytokine profile. BM cells from naïve BALB/c mice were cultured in the presence of mouse rGM‐CSF (20 ng/ml) and/or CT‐26 conditioned media (25% of total volume) for 8 days using an ultra‐low attachment plate. At Day 8, cells were collected, counted, and incubated in the presence of LPS (1 μg/ml) for 24 h. Then, cell supernatants were collected, filtered, and assayed for the presence of cytokines and growth factors using the Multiplex assay. *P < 0.05.