FIG. 6.
Cell-type-specific activation of FRNK expression. (A) RNase protection of total RNA from CEF and B-cell lymphoma cell line BK3A shows lack of FRNK expression in BK3A cells. RNase protection experiments were carried out with RNA purified from CEF and BK3A cells, using probes specific for FAK and FRNK as depicted in Fig. 1. (B) Hind8.3-1 fails to drive luciferase activity in BK3A cells. Transfections were carried out as for Fig. 5. Numbers denote fold activation over pGL3B. (C) The SV40 enhancer does not significantly enable Hind8.3-1 to drive luciferase expression in BK3A cells. Numbers above error bars represent fold activation over pGL3E; error bars denote standard deviations from the means of three separate transfection events.