FIG. 1.
cdc5p is a 97 kDa nuclear protein. Anti-cdc5p antibodies recognize a protein of 97 kDa from Tran[35S]-labeled S. pombe lysates (A and B) and from in vitro-produced cdc5p (A). (A) Autoradiograph of in vitro-produced cdc5p and immunoprecipitates from cells metabolically labeled with Tran[35S]-label by using immune and preimmune sera. (B) Immunoblot of anti-cdc5p immunoprecipitations from cells containing no plasmid, a plasmid encoding a 215-amino-acid truncation of cdc5p, and a plasmid encoding a 429-amino-acid truncation of the carboxy terminus of cdc5p. (C to H) Cdc5p is localized to the nucleus by indirect immunofluorescence. (C) Indirect immunofluorescence performed on wild-type S. pombe cells fixed with methanol-formaldehyde and probed with anti-cdc5p polyclonal antibodies. (D) DAPI staining of field in panel C. (E) Indirect immunofluorescence with preimmune sera. (F) DAPI staining of field in panel E. (G) Indirect immunofluorescence with 12CA5 in the cdc5HA strain. (H) 12CA5 staining of wild-type cells. (Panels G and H were exposed for the same length of time.)