FIG. 4.
(A) Rod1 expression in various organs of rats at 5 weeks of age. (B) Rod1 expression in various organs in early stages of development. Lysates (10 μg of protein) prepared from various tissues of embryonic (E) and postnatal (P) rats were separated by SDS–8% PAGE, and the Rod1 protein was detected by Western blotting with the anti-human Rod1 monoclonal antibody 2B3. As a reference, β-actin protein was detected by reimmunoblotting the same membrane filters that were used for Rod1 detection. The asterisks indicate tissues expressing muscle-type (non-β) actin relatively abundantly (18, 54). Two arrowheads indicate Rod1 proteins migrating as ca. 57- and 50-kDa bands.