FIG. 6.
Kaiso is ubiquitously expressed. (A) Northern blot analysis of Kaiso expression in tissues. A murine multiple tissue Northern blot (Clontech) was probed with a Kaiso cDNA probe (nt 870 to 2583) and washed under high stringency. A Kaiso mRNA of approximately 5 to 6 kb was ubiquitously expressed, and the lowest levels were found in the brain and testis. (B) Western blot analysis of Kaiso protein expression in various cell types. Whole-cell lysates of each cell line were normalized for equal protein amounts, immunoprecipitated with Kaiso MAb 6F8, and detected by Western blotting with a Kaiso polyclonal antibody (pAb). Cell lines are indicated at the top of the panel. Kaiso migrates as an ∼95-kDa doublet in most cells, but interestingly rodent Kaiso (NIH 3T3, CHO, MLL, AT2, and MC26 cell lines) migrated as a slightly larger doublet (∼110 kDa).