FIG. 5.
Subcellular localization of Kaiso in MDCK and NIH 3T3 cells by immunofluorescence. Endogenous Kaiso was primarily concentrated in the nucleus (A and C) as detected by immunofluorescence with Kaiso polyclonal antibodies. The staining was mostly diffuse (panel A) but was also associated with small punctate dot structures (panel C). Overexpressed Kaiso was efficiently localized to the nucleus (B) as detected by the Kaiso-specific MAb 12H9. However, in some cells, nuclear and cytosolic Kaiso was detected (D).